Research

Innovative research and technique development


TestEasy want to become an innovative research, technique development company focusing on molecular diagnosis for infectious disease or cancer. TestEasy focuses pioneering research and will be spin-out company from the laboratory.

Research Areas

Genetic diseases or cancers


Due to various factors such as environmental pollution and changes in dietary structure, the incidence of tumors has continued to rise in recent years. Almost everyone and every family is directly or indirectly attacked and harassed by cancer. In the face of such a current situation and huge challenges, mobilizing the whole society to actively participate in cancer prevention and early diagnosis and treatment is an effective way to reduce the incidence of cancer and increase the cure rate of cancer.


Human disease symptoms are complex and unspecific, but knowledge regarding the links between genetic variants and many different diseases including cancer is growing, therefore genetics analysis will be indispensable in routine diagnostics. However, it is not always easy to choose the most effective diagnostic strategy. Our team will consist of experts with many years of experience in human genetic diagnostics. After collecting biomarker information or further NGS data analysis, our team is available to support the physician in choosing the diagnostic strategy for individual patients and to discuss the results of the analysis.



Breat Cancer

Breast cancer is a malignant tumor that occurs in the epithelial tissue of the breast, and it develops from breast tissue. 99% of breast cancers occur in women, while men account for only 1%.

Breast cancer has become a major public health problem in the current society, and its cause is not yet fully understood. Studies have found that there is a certain regularity in the incidence of breast cancer, and women with high risk factors for breast cancer are prone to breast cancer.


In February 2021, WHO cancer experts stated that among cancers diagnosed in 2020, breast cancer has become the most common cancer in the world.

01

Cervical Cancer

Usually called uterine cancer actually refers to endometrial carcinoma (endometrial carcinoma), also known as uterine corpus cancer, which occurs in the epithelium of the uterus, most of which are adenocarcinomas. It is a common cancer in the female reproductive system. Its incidence is second only to cervical cancer and ranks fourth among the most common cancers in women. It is more common in women between the ages of 50 and 60, and it often occurs after menopause. In recent years, the incidence rate has been on the rise worldwide.


According to clinical statistics, about 20% of patients with endometrial cancer have a family history, and their family history is twice as high as that of cervical cancer.

02

Oral Cancer

Oral cancer is the general term for malignant tumors that occur in the oral cavity. 90% are squamous cell carcinomas. The oral cavity includes the lips, buccal mucosa (the lining of the lips and cheeks), gums, tongue, floor of the mouth, jaw, and gums. And the small area behind the molars. Oral cancer ranks high among various cancers of the whole body. Early detection is critical for the prognosis of oral cancer. If this is detected early, the 5-year survival rate can reach to 70-90%


We will develop a kit for oral cancer detection. To study nucleic acid aptamers, the changes in affinity and content of human BPIFB2 (BPI Fold Containing Family B Member 2) protein, and the expression of SATB1 (special AT-rich sequence binding protein 1) to detect oral cancer. Oral preservation fluid can quickly break down mucus and lyse blood. It is prepared into a corresponding kit, which will be used for oral cancer screening with a special sampling brush for non-invasive sampling, which is suitable for oral cancer and oral precancerous detection and diagnosis. It is mainly suitable for oral precancerous lesions, early diagnosis of asymptomatic oral precancerous lesions, early diagnosis of tumors, population surveys, efficacy monitoring, or early oral cancers with unclear lesion scope.

03


Urine testing




Urine testing may be as effective as the smear test at preventing cervical cancer and could significantly increase participation rates for screening。



Researchers have found a protein in urine that causes breast cancer cells to spread. They have also found the substance in the urine of women with malignant breast cancer. Urine is a painless test. This protein and the gene that controls it are called lipocalin 2 or Lcn2, and they promote the spread of cancer cells in the body.

04

Prostate Cancer

Prostate cancer is the second leading cause of cancer mortality in men. Almost all prostate cancers develop from glandular cells.

The prostate specific antigen (PSA) test can help detect prostate cancer, but it cannot provide an accurate diagnosis.

Notally, eating more dairy products and meat in the diet is associated with a higher risk of disease, while eating more fish or tomato products is associated with a lower prevalence.

05

Colon Cancer

Colon cancer is a common malignant tumor of the digestive tract that occurs in the colon. It is more likely to occur at the junction of the rectum and the sigmoid colon. The incidence is highest in the 40-50-year-old age group, and the ratio of male to female is 2 to 3:1.

Currently, colonoscopy is still the gold standard diagnostic test for CRC detection. Nevertheless, this technique is invasive and expensive.

Gene methylation detection


Gene methylation detection (PCR fluorescent probe method) consists of 2 steps. In the first step, the free DNA is extracted from the plasma with a plasma extraction reagent and then transformed with sulfite; in the second step, the sulfite-converted DNA (bisDNA) is subjected to multiple PCR amplification with PCR reagents. In order to detect the methylated Septin9 gene DNA fragment and the internal control ACTB (ß-actin).

06

Liver Cancer

Liver cancer refers to malignant tumors that occur in the liver. It is the top three cancer in many countries. Liver cancer is a cancer with a high mortality rate. In the early stage, it is easy to be overlooked because of the characteristic of the liver that does not hurt. Common clinical manifestations include liver pain, abdominal distension, anorexia, fatigue, weight loss, progressive hepatomegaly or upper abdominal masses.


The early stage of liver cancer is relatively insidious, and the patients are often in the middle and late stages when they are diagnosed. Early detection, early diagnosis, and early treatment can effectively improve the effect of liver cancer treatment and prolong the lives of liver cancer patients. It promotes the early diagnosis of liver cancer, and the sensitive and accurate qualitative and quantitative detection of liver cancer markers is also an important measure to improve the survival rate of liver cancer patients.
The current early diagnosis of liver cancer such as combined detection and the application of ctDNA (circulating tumor DNA) make it possible to diagnose liver cancer early, but the combined detection costs are high, ctDNA components are small, and the heterogeneity is serious. MicroRNA (miRNA), as a newly discovered gene regulator, has a complex physiological mechanism. The research on microRNA as a disease diagnostic marker is short, and there are no mature microRNA diagnostic products that have officially entered the clinic on a large scale.

Alaska Surical Oncology


Wikimedia Commons 

MicroRNA detection


MicroRNA (miRNA) is a class of non-coding single-stranded RNA molecules with a length of approximately 22 nucleotides encoded by endogenous genes, which are widely present in various organisms from plants, viruses to humans. MicroRNA has a wide range of biological functions. It can specifically bind to mRNA to recruit related RNases and cause mRNA degradation, preventing them from being translated into proteins, thereby affecting its biological functions. MicroRNA plays an important role in tumor suppressor gene inactivation and oncogene activation. effect. In recent years, studies have found that microRNA is closely related to biological phenomena such as the occurrence, development and metastasis of various human malignant tumors, and plays a regulatory role in the above-mentioned biological phenotypes. Scientists have found that microRNA is closely related to cancer (colorectal cancer and other intestinal diseases, liver cancer, etc.). There are obvious differential expressions of microRNA in cancer patients with different prognosis and benign lesions. This differential expression is microRNA as a cancer diagnosis and prognosis. Biomarkers provide feasibility.


Schematic drawing showing the principal of padlock probe recognition of miRNAs with the rolling circle amplification. Based on the particular miRNA sequences, padlock probes are designed to specifically recognize them. The padlock probes annealing to the miRNA are circularized upon addition of DNA ligase. After ligation the annealed miRNA serves as a primer for extension by a phi29 DNA polymerase. The phi29 DNA polymerase facilitates rolling circle amplification, thereby producing a DNA product containing multiple copies of the miRNA sequence.

Research Contact


Peter Gustafsson

PhD


Email: research@testeasy.se